[巯基试剂对大鼠肝脏醛脱氢酶活性的影响]

[Effect of SH-reagents on aldehyde dehydrogenase activity of the rat liver].

作者信息

Konoplitskaia K L, Kuz'mina G I, Grigor'eva M V, Pozniakova T N

出版信息

Ukr Biokhim Zh (1978). 1984 Nov-Dec;56(6):628-33.

Abstract

SH-reagents: tetraethylthiuram disulphide (TETD), 5,5'-dithiobisnitrobenzoic acid (DTNB), p-chloromercurybenzoate (p-ChMB), N-ethylmaleimide (NEM) were studied for their effect on the aldehyde dehydrogenase activity of mitochondrion (isoenzymes I and II) and microsome (isoenzyme II) fractions of the rat liver. TETD is established to inhibit isoenzyme I and isoenzyme II activity of mitochondrial aldehyde dehydrogenase by 100 and 50%, respectively, and the microsomal enzyme activity by 20%. DTNB and NEM inhibit 30-50% of the activity in two isoforms of mitochondrial aldehyde dehydrogenase having no effect on the enzymic activity in microsomes; p-ChMB inhibits completely the activity of the enzyme under study both in the mitochondrial and microsomal fractions. A conclusion is drawn that SH-groups are very essential for manifestation of the catalytic activity in the NAD+-dependent aldehyde dehydrogenase from mitochondrial and microsomal fractions.

摘要

对二乙氨基二硫代甲酸钠（TETD）、5,5'-二硫代双硝基苯甲酸（DTNB）、对氯汞苯甲酸（p-ChMB）、N-乙基马来酰亚胺（NEM）等含硫试剂对大鼠肝脏线粒体（同工酶I和II）和微粒体（同工酶II）部分的醛脱氢酶活性的影响进行了研究。已确定TETD分别抑制线粒体醛脱氢酶的同工酶I和同工酶II活性100%和50%，以及微粒体酶活性20%。DTNB和NEM抑制线粒体醛脱氢酶两种同工型活性的30 - 50%，对微粒体中的酶活性无影响；p-ChMB完全抑制所研究酶在线粒体和微粒体部分的活性。得出的结论是，巯基对于线粒体和微粒体部分依赖NAD +的醛脱氢酶催化活性的表现非常重要。