Radziuk J, McDonald T J, Rubenstein D, Dupre J
Metabolism. 1978 Jun;27(6):657-69. doi: 10.1016/0026-0495(78)90003-3.
Estimates of initial splanchnic uptake of ingested glucose and the concomitant suppression of endogenous glucose production were obtained in man by validated tracer techniques for non--steady-state turnover measurement. Nine normal volunteers (18--44 yr old) fasted overnight received intravenous infusions of tracer (3-3H-glucose or 1-14C-glucose) and a low (45 +/- 1 g) or high (96 +/- 5 g) oral load of glucose labeled with an alternative tracer (1-14C-glucose or 2-2H-glucose). A two-compartment model was used to derive rates of peripheral appearance (Ra) of glucose from all sources (total) and the Ra of ingested glucose. Ra (total glucose) and Ra (ingested glucose) were integrated from the first appearance of ingested glucose until the basal Ra (total glucose) or 116 +/- 6 (SEM) mg/min was reattained. The total amount of glucose reaching the systemic pool in this time was 95 +/- 4 g and 46 +/- 3 g with high and low doses, respectively. Of these quantities 86 +/- 4 g and 40 +/- 3 g originated in the oral glucose, representing 90% +/- 4% of the administered glucose. The remainder (11% +/- 2% of the total) represented endogenous production, suppressed by 66% +/- 6% relative to basal. Sequestration of ingested glucose and subsequent release did not take place during the study since identical results were obtained with ingested 1-14C-glucose or 2-3H-glucose. The latter label would have been lost if the glucose had entered the hexose--phosphate pool. Thus, in normal man approximately 90% of an ingested glucose load is absorbed and passes through the liver to appear in the systemic pool.
采用经过验证的非稳态周转测量示踪技术，在人体中获得了摄入葡萄糖的初始内脏摄取量以及内源性葡萄糖生成的相应抑制情况。9名正常志愿者（年龄18 - 44岁）隔夜禁食，接受静脉输注示踪剂（3 - 3H - 葡萄糖或1 - 14C - 葡萄糖）以及口服低剂量（45±1克）或高剂量（96±5克）用另一种示踪剂（1 - 14C - 葡萄糖或2 - 2H - 葡萄糖）标记的葡萄糖。使用两室模型得出所有来源葡萄糖的外周出现率（Ra）（总量）以及摄入葡萄糖的Ra。从摄入葡萄糖首次出现直至恢复基础Ra（总葡萄糖）或116±6（SEM）毫克/分钟，对Ra（总葡萄糖）和Ra（摄入葡萄糖）进行积分。在此期间，高剂量和低剂量情况下分别有95±4克和46±3克葡萄糖进入全身循环池。其中，86±4克和40±3克源于口服葡萄糖，分别占所给予葡萄糖的90%±4%。其余部分（占总量的11%±2%）代表内源性生成，相对于基础水平被抑制了66%±6%。由于摄入1 - 14C - 葡萄糖或2 - 3H - 葡萄糖得到相同结果，所以在研究过程中未发生摄入葡萄糖的隔离及随后释放的情况。如果葡萄糖进入了己糖磷酸池，后一种标记将会丢失。因此，在正常人体中，摄入的葡萄糖负荷约90%被吸收并经肝脏进入全身循环池。
