Wahlund L O, Sääf J, Ross S B, Wetterberg L, Smedby Y
Acta Physiol Scand. 1984 Oct;122(2):97-102. doi: 10.1111/j.1748-1716.1984.tb07487.x.
Monoamine oxidase in platelets collected on two different occasions, from twelve apparently healthy subjects, were studied. The influence of human platelet poor plasma (PPP) on MAO thermolability and variation in enzyme activity over time was investigated. A 30-70% reduction of the activity was observed after heat treatment at 52 degrees C for 30 min. Activity of MAO was determined before (C) and after heat treatment (H). No significant differences between the H/C ratios on the two occasions were observed for either Vo, Vmax or Km with or without 10% PPP added to the incubates. A temporal increase of the kinetic parameters was observed. The magnitude of this increase was smaller for vo than for apparent Km and Vmax. Platelet MAO activity regulation by human PPP is discussed.
对12名明显健康的受试者在两个不同时间采集的血小板中的单胺氧化酶进行了研究。研究了人少血小板血浆（PPP）对MAO热稳定性以及酶活性随时间变化的影响。在52℃热处理30分钟后，观察到活性降低了30 - 70%。在热处理前（C）和热处理后（H）测定MAO活性。在孵育液中添加或不添加10% PPP的情况下，两次测量的H/C比值在Vo、Vmax或Km方面均未观察到显著差异。观察到动力学参数随时间增加。vo的增加幅度小于表观Km和Vmax。讨论了人PPP对血小板MAO活性的调节作用。
