Kjeldstad B, Johnsson A, Sandberg S
Arch Dermatol Res. 1984;276(6):396-400. doi: 10.1007/BF00413361.
Propionibacterium acnes was grown on Eagle's medium for 4-15 days at pH 5.3-7.2 The porphyrin production was measured both by direct fluorometry and by high pressure liquid chromatography (HPLC). The greatest concentration of porphyrins was produced at pH 6.1. Protoporphyrin was the dominant porphyrin species present in the bacteria in all samples. The relative amount of coproporphyrin was greatest at pH 6.7 after 4 days of incubation. In human skin there are local variations in the pH; therefore our findings may be of importance for porphyrin production in acne.
痤疮丙酸杆菌在伊格尔培养基上于pH 5.3 - 7.2条件下培养4 - 15天。通过直接荧光法和高压液相色谱法（HPLC）测定卟啉产量。在pH 6.1时产生的卟啉浓度最高。原卟啉是所有样品中细菌内存在的主要卟啉种类。在培养4天后，粪卟啉的相对含量在pH 6.7时最高。人体皮肤的pH存在局部差异；因此我们的研究结果可能对痤疮中卟啉的产生具有重要意义。
