Lee W L, Shalita A R, Poh-Fitzpatrick M B
J Bacteriol. 1978 Feb;133(2):811-5. doi: 10.1128/jb.133.2.811-815.1978.
Porphyrin production by Propionibacterium acnes and that by Propionibacterium granulosum were compared. Porphyrin synthesized by both organisms was identified as coproporphyrin III on the bases of absorption and fluorescence spectra and behavior on paper chromatography and thin-layer chromatography. Quantitative, rather than qualitative, differences in production were found between these organisms. In general, P. granulosum produced significantly greater amounts (P less than 0.001) of porphyrin than did P. acnes. delta-Aminolevulinic acid synthetase appeared to be the rate-limiting enzyme of the heme biosynthetic pathway in both organisms. The increased porphyrin production in P. granulosum is apparently associated with increased delta-aminolevulinic acid synthetase activity.
对痤疮丙酸杆菌和颗粒丙酸杆菌产生卟啉的情况进行了比较。根据吸收光谱、荧光光谱以及在纸色谱和薄层色谱上的表现，确定这两种微生物合成的卟啉均为粪卟啉III。发现这两种微生物在卟啉产生量上存在定量而非定性的差异。总体而言，颗粒丙酸杆菌产生的卟啉量显著多于痤疮丙酸杆菌（P<0.001）。δ-氨基乙酰丙酸合成酶似乎是这两种微生物血红素生物合成途径中的限速酶。颗粒丙酸杆菌中卟啉产量的增加显然与δ-氨基乙酰丙酸合成酶活性的增加有关。
