Smeaton T C
Aust J Exp Biol Med Sci. 1984 Aug;62 ( Pt 4):453-63. doi: 10.1038/icb.1984.43.
The kinetics of migration of bone marrow cells to the peritoneal cavity have been studied using in vivo labelling of the marrow of the rat tibia with (methyl-3H)-thymidine (TdR). The incorporation of 3H into DNA is directly related to the amount infused over a thirty-minute period, though only 2-3% of the infused dose is incorporated into DNA in the bone. Autoradiography has shown widespread labelling of bone marrow cells throughout the tibia. Band cells and polymorphonuclear neutrophils (PMN) show an increase in labelling during the 2-3 days after infusion, confirming the maturation of granulocytes prior to their release to the circulation. The disappearance of 3H-labelled DNA from the bone occurs rapidly during the 3 days after infusion of TdR with a half-life (t1/2) of 1.4-1.7 days. There is a concomitant increase in the specific activity of DNA in peritoneal cells elicited by agar, particularly in the macrophage (M phi). Labelling of PMN in the peritoneal exudate occurs only when agar is injected from 2-4 days after infusion of TdR. The time for maturation of PMN in the rat bone marrow (BM) (2-3 days) has been confirmed, and the method of labelling permits both measurement of the migration of cells from BM to peritoneal cavity and an estimate of the rates of migration.
利用（甲基 - ³H）- 胸腺嘧啶核苷（TdR）对大鼠胫骨骨髓进行体内标记，研究了骨髓细胞向腹腔迁移的动力学。³H掺入DNA的量与30分钟内注入的量直接相关，不过注入剂量中只有2 - 3%掺入骨中的DNA。放射自显影显示胫骨内骨髓细胞广泛标记。带状细胞和多形核中性粒细胞（PMN）在注入后2 - 3天标记增加，证实粒细胞在释放到循环之前已经成熟。注入TdR后3天内，³H标记的DNA从骨中迅速消失，半衰期（t1/2）为1.4 - 1.7天。琼脂引发的腹腔细胞中DNA的比活性随之增加，特别是在巨噬细胞（M phi）中。只有在注入TdR后2 - 4天注射琼脂时，腹腔渗出液中的PMN才会被标记。大鼠骨髓（BM）中PMN成熟的时间（2 - 3天）得到了证实，这种标记方法既可以测量细胞从BM向腹腔的迁移，也可以估计迁移速率。
