Schmid H
Basic Appl Histochem. 1984;28(3):221-31.
A simple method for microphotometric evaluation of cryostat sections from human renal tissue routinely stained for succinate dehydrogenase activity by means of tetranitro-blue tetrazolium chloride is described and tested for validity. Manual absorbance measurement within single nephron segments from the same section allows to directly visualize the distribution pattern of this enzyme along the nephron. Photometric data can be expressed in relative enzyme activities by using the cortical collecting ducts within the same section as reference. This allows to compare measurements of different kidney sections stained by various incubation procedures. The agreement found between relative succinate dehydrogenase activities and recently published morphometric data on mitochondrial inner membranes along the rat nephron suggests that quantitative succinate dehydrogenase microphotometry is a useful histochemical tool for the assessment of renal mitochondrial cristae membranes.
本文描述并测试了一种简单的方法，用于对通过氯化四氮唑蓝常规染色以检测琥珀酸脱氢酶活性的人肾组织冷冻切片进行显微光度评估。在同一切片中的单个肾单位节段内进行手动吸光度测量，可以直接观察到该酶沿肾单位的分布模式。通过将同一切片中的皮质集合管作为参考，可以用相对酶活性来表示光度数据。这使得能够比较通过各种孵育程序染色的不同肾脏切片的测量结果。在相对琥珀酸脱氢酶活性与最近发表的关于大鼠肾单位沿线粒体内膜的形态计量学数据之间发现的一致性表明，定量琥珀酸脱氢酶显微光度法是评估肾线粒体嵴膜的一种有用的组织化学工具。
