微管相关蛋白MAP2被鸟苷三磷酸磷酸化。
Phosphorylation of the microtubule-associated protein MAP2 by GTP.
作者信息
Burns R G, Islam K
出版信息
Biochem J. 1984 Dec 1;224(2):623-7. doi: 10.1042/bj2240623.
Abstract
The chick brain microtubule-associated protein MAP2 can be phosphorylated in vitro to the extent of 12 mol/mol with GTP at the same sites as can be labelled by the cyclic AMP-independent protein kinase utilizing [gamma-32P]ATP as the phosphoryl donor. Consequently, the microtubule protein is chemically modified by the conditions usually employed for studies of microtubule assembly, so that the derived kinetic parameters may not relate to steady-state conditions.
摘要
鸡脑微管相关蛋白MAP2在体外可被磷酸化，磷酸化程度可达12摩尔/摩尔，磷酸化位点与环磷酸腺苷非依赖性蛋白激酶利用[γ-32P]ATP作为磷酰供体进行标记的位点相同。因此，微管蛋白会因通常用于微管组装研究的条件而发生化学修饰，这样得出的动力学参数可能与稳态条件无关。
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