卡巴胆碱和银杏叶提取物对兔离体主动脉的内皮依赖性舒张作用。

Endothelium-dependent relaxations of rabbit isolated aorta produced by carbachol and by Ginkgo biloba extract.

作者信息

Delaflotte S, Auguet M, DeFeudis F V, Baranes J, Clostre F, Drieu K, Braquet P

出版信息

Biomed Biochim Acta. 1984;43(8-9):S212-6.

Abstract

Spirally-cut strips of rabbit aorta were used to examine the relaxations produced by carbachol and extract of Ginkgo biloba (Gb) under isometric conditions. After precontracting the strips with phenylephrine (10(-7) M), carbachol produced a dose-related relaxation (PD2 congruent to 6.2 +/- 0.1) and this effect was antagonized competitively by atropine (PA2 congruent to 9.4 +/- 0.1). Gb (0.2 or 0.3 mg/ml) also relaxed the strips. Removal of the endothelium or a 30-min pre-treatment of the strips with a substance that has lipoxygenase-inhibitor activity (nordihydroguaiaretic acid, NDGA, 10(-5) M) abolished the relaxant effect of carbachol and partially blocked the relaxant effect of Gb. Thus, at least part of the relaxant effect of Gb is mediated by a factor(s) (e.g., EDRF) that is released from endothelial cells.

摘要

采用螺旋状切割的兔主动脉条，在等长条件下检测卡巴胆碱和银杏叶提取物（Gb）引起的舒张作用。在用去氧肾上腺素（10⁻⁷ M）使主动脉条预收缩后，卡巴胆碱产生剂量相关的舒张作用（pD₂约为6.2±0.1），且该作用被阿托品竞争性拮抗（pA₂约为9.4±0.1）。Gb（0.2或0.3 mg/ml）也使主动脉条舒张。去除内皮或用具有脂氧合酶抑制活性的物质（去甲二氢愈创木酸，NDGA，10⁻⁵ M）对主动脉条进行30分钟预处理，可消除卡巴胆碱的舒张作用，并部分阻断Gb的舒张作用。因此，Gb的舒张作用至少部分是由内皮细胞释放的一种或多种因子（如内皮舒张因子）介导的。