Ogiso M, Ohnishi E
Mol Cell Endocrinol. 1984 Nov;38(1):13-9. doi: 10.1016/0303-7207(84)90140-0.
Uptake of ecdysone by ovaries of the silkworm, Bombyx mori, was studied by organ culture. [3H]Ecdysone was transported almost linearly into the ovary for up to 3 h of the incubation. The uptake was proportional to the concentration of the labeled ecdysone, unsaturably, at concentrations ranging up to 10(-6) M. Ecdysone which had been transported into the ovary could usually be removed when the ovary was re-transferred to the ecdysone-free medium. Analysis of the transported compounds by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed the marked conversion of ecdysone into 20-hydroxyecdysone, unknown metabolites and conjugated forms. Physiological significance of the metabolic activity of the ovary is discussed with respect to the accumulation of ecdysteroids in the ovary.
通过器官培养研究了家蚕卵巢对蜕皮激素的摄取。在长达3小时的孵育过程中，[3H]蜕皮激素几乎呈线性转运到卵巢中。摄取量与标记蜕皮激素的浓度成正比，在浓度高达10(-6)M的范围内不饱和。当卵巢重新转移到不含蜕皮激素的培养基中时，已转运到卵巢中的蜕皮激素通常可以被去除。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）对转运化合物的分析表明，蜕皮激素显著转化为20-羟基蜕皮激素、未知代谢物和结合形式。就卵巢中蜕皮甾体的积累而言，讨论了卵巢代谢活性的生理意义。
