Metabolism of ecdysone and 20-hydroxyecdysone in adult Drosophila melanogaster.

The metabolism of [3H]ecdysone injected into adult female and male Drosophila melanogaster was investigated. The metabolites present in flies and faeces were analysed separately after incubation times of 1, 2 or 4 h. In female flies ecdysone-22-fatty acid acyl esters were the major metabolites followed by 3-dehydroecdysone, 26-hydroxyecdysone, ecdysonoic acid, 20-hydroxyecdysone and a negatively charged conjugate of ecdysone. In male flies the same compounds were formed, but their relative concentrations were somewhat different from those in female flies. All metabolites formed can be excreted. [3H]20-hydroxyecdysone was metabolized in much the same way: 20-hydroxyecdysone-22-acyl esters, 3-dehydro-20-hydroxyecdysone, 20-hydroxy-ecdysonoic acid and a negatively charged conjugate of 20-hydroxyecdysone were formed. However, 20,26-dihydroxyecdysone could not be detected after injection of [3H]20-hydroxyecdysone.