Grau V, Lafont R
ENS, Département de Biologie, CNRS URA 686, Paris, France.
Insect Biochem Mol Biol. 1994 Jan;24(1):49-58. doi: 10.1016/0965-1748(94)90122-8.
The metabolism of [3H]ecdysone injected into adult female and male Drosophila melanogaster was investigated. The metabolites present in flies and faeces were analysed separately after incubation times of 1, 2 or 4 h. In female flies ecdysone-22-fatty acid acyl esters were the major metabolites followed by 3-dehydroecdysone, 26-hydroxyecdysone, ecdysonoic acid, 20-hydroxyecdysone and a negatively charged conjugate of ecdysone. In male flies the same compounds were formed, but their relative concentrations were somewhat different from those in female flies. All metabolites formed can be excreted. [3H]20-hydroxyecdysone was metabolized in much the same way: 20-hydroxyecdysone-22-acyl esters, 3-dehydro-20-hydroxyecdysone, 20-hydroxy-ecdysonoic acid and a negatively charged conjugate of 20-hydroxyecdysone were formed. However, 20,26-dihydroxyecdysone could not be detected after injection of [3H]20-hydroxyecdysone.
对注射到成年雌性和雄性黑腹果蝇体内的[³H]蜕皮激素的代谢进行了研究。在1、2或4小时的孵育时间后，分别分析果蝇和粪便中存在的代谢产物。在雌性果蝇中，蜕皮激素-22-脂肪酸酰酯是主要代谢产物，其次是3-脱氢蜕皮激素、26-羟基蜕皮激素、蜕皮甾酮酸、20-羟基蜕皮激素和一种带负电荷的蜕皮激素结合物。在雄性果蝇中也形成了相同的化合物，但它们的相对浓度与雌性果蝇中的有所不同。形成的所有代谢产物都可以排出体外。[³H]20-羟基蜕皮激素的代谢方式大致相同：形成了20-羟基蜕皮激素-22-酰酯、3-脱氢-20-羟基蜕皮激素、20-羟基-蜕皮甾酮酸和一种带负电荷的20-羟基蜕皮激素结合物。然而，注射[³H]20-羟基蜕皮激素后未检测到20,26-二羟基蜕皮激素。
