Hertl W, Ramsey W S, Nowlan E D
In Vitro. 1984 Oct;20(10):796-801. doi: 10.1007/BF02618295.
A centrifugal method has been evaluated for measuring the strength of Vero Green Monkey kidney cell adhesion to growth surfaces. The centrifugal force necessary to remove cells gave a quantitative measure of cell adhesion and hence the quality of the growth surface. After being subjected to high gravity forces, both the remaining attached cells and the detached cells were viable, indicating the detachment process did not simply rupture the cell. Electron microscope examination of growth surfaces after cell detachment suggested that remnants related to filopodia remained.
已对一种离心方法进行了评估，用于测量非洲绿猴肾细胞（Vero细胞）对生长表面的黏附强度。去除细胞所需的离心力给出了细胞黏附的定量指标，从而反映出生长表面的质量。在经受高重力作用后，剩余的贴壁细胞和脱离的细胞均具有活力，这表明细胞脱离过程并非简单地使细胞破裂。对细胞脱离后的生长表面进行电子显微镜检查发现，与丝状伪足相关的残留物依然存在。
