抗半乳糖基抗体与半乳糖基化脂质体的结合。

Binding of anti-galactosyl antibodies to galactosylated liposomes.

作者信息

Sarkar D P, Das M K

出版信息

Immunol Lett. 1984;8(5):257-60. doi: 10.1016/0165-2478(84)90005-1.

PMID:6519682

Abstract

The binding characteristics of rabbit anti-galactosyl antibodies elicited through galactosylated liposomes were studied. The association constants (13 X 10(5) M-1 and 21 X 10(5) M-1 of Fabmu and Fab fragments with the galactosylated liposomes were determined by measuring the binding of radioiodinated antibodies. The effect of free ligand (methyl-beta-D-galactopyranoside) and the ligand coupled to the liposomal surface (galactosylated liposomes) on the structure of antibodies was investigated using circular dichroism (CD) measurements. Changes were observed in both the secondary and tertiary structure of antibodies upon binding to galactosylated liposomes. In contrast, the free ligand induced alterations only in the near-UV region of the CD spectra, indicating a change in tertiary structure only.

摘要

研究了通过半乳糖化脂质体引发的兔抗半乳糖基抗体的结合特性。通过测量放射性碘化抗体的结合，确定了Fabμ和Fab片段与半乳糖化脂质体的缔合常数（分别为13×10⁵ M⁻¹和21×10⁵ M⁻¹）。使用圆二色性（CD）测量研究了游离配体（甲基-β-D-吡喃半乳糖苷）和偶联到脂质体表面的配体（半乳糖化脂质体）对抗体结构的影响。在抗体与半乳糖化脂质体结合时，观察到抗体的二级和三级结构均发生变化。相比之下，游离配体仅在CD光谱的近紫外区域引起变化，表明仅三级结构发生了变化。