小鼠脾脏中GM1b的特征分析。

Characterization of GM1b in mouse spleen.

作者信息

Nakamura K, Hashimoto Y, Suzuki M, Suzuki A, Yamakawa T

出版信息

J Biochem. 1984 Oct;96(4):949-57. doi: 10.1093/oxfordjournals.jbchem.a134954.

DOI:10.1093/oxfordjournals.jbchem.a134954

PMID:6520127

Abstract

Two GM1b gangliosides (IV-sialosylgangliotetraosylceramide) containing either N-glycolylneuraminic acid of N-acetylneuraminic acid at the terminal galactose of gangliotetraosylceramide were found in the ICR mouse spleen. Their structures were characterized by the behavior on thin layer chromatograms, sugar composition, susceptibility to sialidase and immunobinding activity toward anti-gangliotetraosylceramide antibody. The structure of GM1b containing N-glycolylneuraminic acid was further confirmed by methylation analysis. GM1 gangliosides containing either N-glycolylneuraminic acid or N-acetylneuraminic acid were also purified and characterized by thin layer chromatography, sugar analysis and sialidase treatment. As a result, the presence of four kinds of monosialoganglioside with a gangliotetraosyl core structure, GM1(NeuAc), GM1(NeuGc), GM1b(NeuAc), and GM1b(NeuGc), were found to exist in the ICR mouse spleen. These four gangliosides accounted for about 50% of the spleen monosialoganglioside content. Additional four gangliosides in the monosialoganglioside fraction were tentatively characterized as GM3(NeuAc), GM3(NeuGc), GM2(NeuGc), and sialosylneolactotetraosylceramide(NeuGc).

摘要

在ICR小鼠脾脏中发现了两种GM1b神经节苷脂（IV - 唾液酸神经节四糖神经酰胺），它们在神经节四糖神经酰胺的末端半乳糖上分别含有N - 羟乙酰神经氨酸或N - 乙酰神经氨酸。通过薄层色谱行为、糖组成、对唾液酸酶的敏感性以及对抗神经节四糖神经酰胺抗体的免疫结合活性对其结构进行了表征。含有N - 羟乙酰神经氨酸的GM1b结构通过甲基化分析进一步得到证实。还纯化了含有N - 羟乙酰神经氨酸或N - 乙酰神经氨酸的GM1神经节苷脂，并通过薄层色谱、糖分析和唾液酸酶处理对其进行了表征。结果发现，在ICR小鼠脾脏中存在四种具有神经节四糖核心结构的单唾液酸神经节苷脂，即GM1（NeuAc）、GM1（NeuGc）、GM1b（NeuAc）和GM1b（NeuGc）。这四种神经节苷脂约占脾脏单唾液酸神经节苷脂含量的50%。单唾液酸神经节苷脂组分中的另外四种神经节苷脂初步鉴定为GM3（NeuAc）、GM3（NeuGc）、GM2（NeuGc）和唾液酸新乳糖四糖神经酰胺（NeuGc）。