Radioimmunoassay of 5-methyl-2'-deoxycytidine. A method for the quantitation of DNA methylation.
作者信息
Vilpo J A, Rasi S, Vilpo L M
出版信息
J Immunol Methods. 1984 Dec 31;75(2):241-6. doi: 10.1016/0022-1759(84)90107-8.
PMID:6520398
Abstract
5-Methyl-2'-deoxycytidine (5MedCyd) is a minor constituent of mammalian cell DNA. We report the production and characterization of highly specific rabbit anti-5MedCyd antiserum. The antiserum was suitable for the radioimmunological measurement of 5MedCyd. This simple radioimmunoassay was capable of quantitating calf thymus DNA methylation at nanomolar levels of total DNA.
摘要
Zotero 插件