一种用于定量DNA甲基化的改良放射免疫测定法。
An improved radioimmunoassay for the quantitation of DNA methylation.
作者信息
Vilpo J A, Rasi S, Suvanto E, Vilpo L M
出版信息
J Immunol Methods. 1986 Mar 13;87(2):179-83. doi: 10.1016/0022-1759(86)90528-4.
A novel radioimmunoassay of 5MedCyd is described. The assay, employing a highly specific antiserum raised in rabbits against BSA-conjugated 5MeCyd, used 5-125iodo-2'-deoxycytidine as the tracer. The measuring range for the assay was found to be 1-1000 pmol per assay of 5MedCyd. When the methods were applied to the measurement of methylation in DNA samples a good correlation between the results obtained with the radioimmunoassay and HPLC was demonstrated. The method has several advantages over the more laborious and sophisticated techniques previously available: high sensitivity, large assay range, rapidity and potential for large number of simultaneous assays, simplicity, and low cost provided that the laboratory has equipment for gamma counting.
描述了一种新型的5-甲基胞苷放射免疫测定法。该测定法使用在兔子体内产生的针对牛血清白蛋白偶联的5-甲基胞苷的高度特异性抗血清，以5-¹²⁵碘-2'-脱氧胞苷作为示踪剂。发现该测定法的测量范围为每次测定5-甲基胞苷1-1000皮摩尔。当将这些方法应用于DNA样品甲基化的测量时，放射免疫测定法和高效液相色谱法获得的结果之间显示出良好的相关性。与以前可用的更费力且复杂的技术相比，该方法具有几个优点：高灵敏度、大测定范围、快速性以及大量同时测定的潜力、简单性，并且只要实验室拥有γ计数设备，成本就较低。
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