Baroin A, Garcia-Romeu F, Lamarre T, Motais R
J Physiol. 1984 Nov;356:21-31. doi: 10.1113/jphysiol.1984.sp015450.
The addition of isoprenaline to an isotonic suspension of red blood cells of rainbow trout induces an amiloride-sensitive Na+ transport which is independent of Cl- and insensitive to 4,4'-diisothiocyano-2,2'-stilbene disulphonic acid (DIDS) and furosemide. Na+ uptake is accompanied by amiloride-sensitive H+ release. The H+ efflux is dependent upon the external Na+ concentration, the K0.5 value for Na+ being 16 mM. In the presence of DIDS, when the coupled NaCl entry (NaCl co-transport) induced by catecholamine is blocked, the results provide evidence for a linked movement of Na+ and H+, with a stoicheiometry of 1:1. Exchange of H+ for Na+ induces osmotic swelling of the cells which is due to the replacement of a bound proton by an osmotically active Na+ cation. In the absence of DIDS when the bulk of the Na+ uptake is the result of a coupled entry of Na+ and Cl-, H+ extrusion still occurs and the magnitude of acid excretion is identical to that found in DIDS-treated cells. This suggests that Na+-H+ exchange remains active. Addition of isoprenaline first stimulates the Na+-H+ exchange but only transiently. This is followed by a more permanent stimulation of the NaCl co-transport.
在虹鳟红细胞的等渗悬浮液中添加异丙肾上腺素会诱导一种对氨氯吡咪敏感的Na⁺转运，该转运不依赖于Cl⁻，且对4,4'-二异硫氰基-2,2'-芪二磺酸（DIDS）和呋塞米不敏感。Na⁺摄取伴随着对氨氯吡咪敏感的H⁺释放。H⁺外流取决于外部Na⁺浓度，Na⁺的半最大效应浓度（K0.5）值为16 mM。在DIDS存在的情况下，当儿茶酚胺诱导的耦合NaCl内流（NaCl共转运）被阻断时，结果为Na⁺和H⁺的关联运动提供了证据，化学计量比为1:1。H⁺与Na⁺的交换诱导细胞渗透肿胀，这是由于具有渗透活性的Na⁺阳离子取代了结合的质子。在没有DIDS的情况下，当大部分Na⁺摄取是Na⁺和Cl⁻耦合内流的结果时，H⁺外排仍然发生，酸排泄量与在DIDS处理的细胞中发现的相同。这表明Na⁺-H⁺交换仍然活跃。添加异丙肾上腺素首先刺激Na⁺-H⁺交换，但只是短暂的。随后是对NaCl共转运的更持久刺激。
