Hasin Y, Doorey A, Barry W H
J Mol Cell Cardiol. 1984 Nov;16(11):1009-21. doi: 10.1016/s0022-2828(84)80014-0.
The early electrophysiological and mechanical effects of metabolic inhibition of high energy phosphate production were studied in cultured chick embryo heart cells. Selective inhibition of either glycolysis by 2-deoxyglucose in the presence of acetate or of oxidative phosphorylation by cyanide showed different effects. 2-deoxyglucose induced pronounced reduction in maximal diastolic potential and prolongation of excitation contraction delay, with only a moderate decrease of contractility and with only minimal changes in action potential duration. Cyanide, on the other hand, induced a profound negative inotropic effect and caused slowing of relaxation, shortening of action potential duration, a decrease in the upstroke of the action potential, and only a moderate decrease in the diastolic membrane potential. Exposure to 2-deoxyglucose and cyanide combined produced effects consistent with inhibition of both metabolic pathways. These observations are consistent with the hypothesis that these two metabolic pathways may have specific roles in fueling several energy-demanding functions of the myocardial cell.
在培养的鸡胚心脏细胞中研究了高能磷酸生成代谢抑制的早期电生理和机械效应。在乙酸盐存在的情况下，用2-脱氧葡萄糖选择性抑制糖酵解，或用氰化物抑制氧化磷酸化，显示出不同的效应。2-脱氧葡萄糖导致最大舒张电位明显降低，兴奋-收缩延迟延长，收缩力仅适度降低，动作电位时程仅有微小变化。另一方面，氰化物引起严重的负性肌力作用，导致舒张减慢，动作电位时程缩短，动作电位上升支降低，舒张膜电位仅适度降低。同时暴露于2-脱氧葡萄糖和氰化物产生的效应与两种代谢途径的抑制作用一致。这些观察结果与以下假设一致，即这两种代谢途径可能在为心肌细胞的几种能量需求功能提供能量方面具有特定作用。
