Akaishi T
Nihon Geka Gakkai Zasshi. 1984 Nov;85(11):1440-53.
Seven cell lines have been established from specimens of esophageal cancer bearing patients and examined as to following characteristics. These cells showed various degree of keratinization from scarce tonofilaments to abundant tonofilament bundles within cytoplasm. The ability of tonofilament formation in vitro corresponded well with cancer pearl formation in vivo. but TE-3 strain did not produce tonofilaments whereas it showed marked cancer pearl formation when transplanted to hairless mouse. Consumption of amino acid, arginine, was common to all cell lines and accumulation of glycine and amino acids constituting urea cycle was also common. Serine, aspartic acid and branched chain amino acids were consumed in varying degrees, depending upon the cell line. Especially serine consuming cell line did not consume aspartic acid and vice versa. These seven cell lines showed considerable variety of survival curves for the various agents. These reactions to the drugs seemed to be "individual" and rather independent of the histopathological classification. In conclusion, a fixed method of therapy for cases of esophageal cancer does not seem to be indicated because of the considerable variety in their characteristics of the cancer cells in each patient.
已从食管癌患者的标本中建立了七种细胞系，并对其以下特征进行了检测。这些细胞在细胞质中显示出从稀少的张力丝到丰富的张力丝束的不同程度的角化。体外张力丝形成能力与体内癌珠形成情况良好对应。但是TE - 3株不产生张力丝，而将其移植到无毛小鼠时却显示出明显的癌珠形成。所有细胞系都共同消耗氨基酸精氨酸，并且甘氨酸和构成尿素循环的氨基酸积累也很常见。丝氨酸、天冬氨酸和支链氨基酸的消耗程度各不相同，这取决于细胞系。特别是消耗丝氨酸的细胞系不消耗天冬氨酸，反之亦然。这七种细胞系对各种药物显示出相当多样的存活曲线。这些对药物的反应似乎是“个体性的”，并且相当独立于组织病理学分类。总之，由于每位患者癌细胞特征差异很大，似乎不存在针对食管癌病例的固定治疗方法。
