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[不同浓度去污剂对多头绒泡菌有丝分裂周期中染色质结构影响的研究]

[Study of the effect of different concentrations of a detergent on the chromatin structure of Physarum polycephalum during the mitotic cycle].

作者信息

Pavlov S B, Beliaeva N N, Tikhonenko A S

出版信息

Mol Biol (Mosk). 1984 Nov-Dec;18(6):1461-5.

PMID:6521732
Abstract

The influence of different concentrations of detergent Joy on the chromatin structure of Physarum polycephalum in the process of mitotic cycle was studied electronmicroscopically. The investigations showed that at Joy concentrations less than 0,01% a small part of nuclei disrupt and, as a rule, chromatin is insufficiently dispersed; at concentrations more than 0,1% the detergent may influence the chromatin structure of Physarum polycephalum. Based on the data obtained we consider that the optimal detergent concentrations that practically do not influence the chromatin structure and lead to disruption of the majority of nuclei and to the proper dispersion of chromatin is 0,1-0,01%.

摘要

利用电子显微镜研究了不同浓度的洗洁精“快乐洁肤”在有丝分裂周期过程中对多头绒泡菌染色质结构的影响。研究表明,当“快乐洁肤”浓度低于0.01%时,一小部分细胞核会破裂,而且通常染色质分散不充分;当浓度高于0.1%时,该洗洁精可能会影响多头绒泡菌的染色质结构。根据所获得的数据,我们认为实际上不影响染色质结构、能导致大多数细胞核破裂并使染色质适当分散的最佳洗洁精浓度为0.1% - 0.01%。

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Mol Biol (Mosk). 1984 Nov-Dec;18(6):1461-5.
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