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Fluorescence labelling of pyrimidine nucleobases and their related compounds for high-performance liquid chromatography.

作者信息

Iwamoto M, Yoshida S, Hirose S

出版信息

Nucleic Acids Symp Ser. 1984(15):21-4.

PMID:6522288
Abstract

Fluorescence derivatization of pyrimidine nucleobases and nucleosides with 4-bromomethyl-7-methoxycoumarin(Br-Mmc) was studied. In dimethylsulfoxide(DMSO) containing powdered potassium carbonate, the reaction completed within 5 min at room temperature. The excess Br-Mmc was treated with p-nitrobenzoic acid(NBA) which gave a non-fluorescent compound. For the determination of serum sample, diethylaminoethyl sepharose(DEAE) column was used in a cleanup procedure.

摘要

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