Aliverdieva D A, Shol'ts K F
Prikl Biokhim Mikrobiol. 1984 Nov-Dec;20(6):823-30.
The mechanism of Coomassie interaction with proteins was being studied. Under certain conditions the range of detectible polypeptides can be wider. For protein analysis in mitochondria we have suggested a new component ratio for the Bradford reagent. A correction for mitochondrial protein was determined, which should be made on using bovine serum albumin as a standard.
正在研究考马斯亮蓝与蛋白质相互作用的机制。在某些条件下,可检测到的多肽范围可能更广。对于线粒体中的蛋白质分析,我们提出了一种新的 Bradford 试剂成分比例。确定了线粒体蛋白质的校正值,在以牛血清白蛋白作为标准时应进行此校正。