de la Cruz M J, Alvarez-Rementería R, Alemany J, Albert P
Rev Esp Fisiol. 1984 Sep;40(3):289-96.
The influence of theophylline and theobromine on cellular respiration and on membrane transport of calcium has been studied in isolated rat liver mitochondria, using oxygen and Ca2+ selective electrodes. A linear decrease in respiratory coefficients, in the total amount and rate of "extra" oxygen consumption induced by ADP is observed with drug concentration. Theobromine does not show any appreciable effect on these respiratory parameters, but this result is similar to that observed with theophylline for the same concentration range. Calcium uptake coupled to respiration is inhibited by both drugs depending on their concentrations. Theobromine is more effective than theophylline. Calcium saturation of the mitochondria takes place in all cases after 36 +/- 2 s but only a 20% of the maximum calcium uptake observed in the absence of the drugs is determined in the presence of 15 mM theophylline or only 1.8 mM theobromine. Comparative studies show direct correlation between the pharmacological activities as stimulants of caffeine, theophylline and theobromine and their behaviour as inhibitors of calcium uptake coupled to respiration by mitochondria.
使用氧电极和钙离子选择性电极，在分离的大鼠肝线粒体中研究了茶碱和可可碱对细胞呼吸以及钙的膜转运的影响。随着药物浓度的增加，观察到呼吸系数、由ADP诱导的“额外”氧气消耗总量和速率呈线性下降。可可碱对这些呼吸参数没有任何明显影响，但在相同浓度范围内，该结果与茶碱观察到的结果相似。两种药物均根据其浓度抑制与呼吸相关的钙摄取。可可碱比茶碱更有效。在所有情况下，线粒体在36±2秒后发生钙饱和，但在存在15 mM茶碱或仅1.8 mM可可碱的情况下，仅测定出在无药物时观察到的最大钙摄取量的20%。比较研究表明，咖啡因、茶碱和可可碱作为兴奋剂的药理活性与其作为线粒体呼吸相关钙摄取抑制剂的行为之间存在直接相关性。
