Alemany J, de la Cruz M J, Roncero I, Miquel J
Department of Biochemistry, School of Medicine, Universidad Complutense, Madrid, Spain.
Exp Gerontol. 1988;23(1):25-34. doi: 10.1016/0531-5565(88)90017-4.
The concept that aging results in an impairment of mitochondrial biochemistry has been tested on organelles isolated from the liver of 3-4-month-old and 24-month-old rats of the Wistar strain. Our data suggest that aging results in significant decreases in succinate-supported respiration, ATP levels and calcium uptake. When theophylline was added to the incubation mixture, both respiration and calcium uptake were depressed in approximately the same proportion in the mitochondria from old rats, although the mitochondrial ATP of young animals was significantly decreased by this substance.
衰老导致线粒体生物化学功能受损这一概念，已在从3 - 4月龄和24月龄Wistar品系大鼠肝脏分离出的细胞器上进行了测试。我们的数据表明，衰老导致琥珀酸支持的呼吸作用、ATP水平和钙摄取显著降低。当将茶碱添加到孵育混合物中时，老龄大鼠线粒体中的呼吸作用和钙摄取均以大致相同的比例受到抑制，尽管该物质会使幼龄动物的线粒体ATP显著降低。
