咖啡因、茶碱和可可碱对大鼠肝脏线粒体钙摄取、呼吸作用及三磷酸腺苷水平的影响

Changes induced by caffeine, theophylline and theobromine on calcium uptake, respiration and ATP levels in rat-liver mitochondria.

de la Cruz M J, Alemany J, Roncero I, Albert P

Departamento de Bioquímica, Facultad de Medicina, Universidad Complutense de Madrid, Spain.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;91(2):443-7. doi: 10.1016/0742-8413(88)90057-6.

Abstract

The inhibition of calcium uptake and cellular respiration depend on the concentration of the compounds as shown by the concentration-effect curves. 2. The concentrations at which 50% inhibition of the transport of calcium takes place (caffeine 45 mM, theophylline 12 mM, theobromine, 4 mM) do not coincide exactly with those that produce the same effect on cellular respiration (caffeine 60 mM, theophylline 22 mM, theobromine 8 mM). 3. ATP concentrations under different conditions were also determined; a decrease in their value induced by the drugs was observed. No significant differences were observed, however, between the effect produced by the methyl-xanthines. 4. These findings suggest that these compounds are able to affect in some way the maintenance of energy gradients linked to the effects studied.

摘要

如浓度效应曲线所示，钙摄取和细胞呼吸的抑制取决于化合物的浓度。2. 钙转运发生50%抑制时的浓度（咖啡因45 mM、茶碱12 mM、可可碱4 mM）与对细胞呼吸产生相同效应时的浓度（咖啡因60 mM、茶碱22 mM、可可碱8 mM）并不完全一致。3. 还测定了不同条件下的ATP浓度；观察到药物导致其值降低。然而，甲基黄嘌呤产生的效应之间未观察到显著差异。4. 这些发现表明，这些化合物能够以某种方式影响与所研究效应相关的能量梯度的维持。