Lok J B, Pollack R J, Cupp E W, Bernardo M J, Donnelly J J, Albiez E J
Tropenmed Parasitol. 1984 Dec;35(4):209-11.
Third-stage larvae of Onchocerca volvulus and O. lienalis were observed to molt to the fourth stage in various cell-free in vitro systems. The percentage of O. lienalis completing the molt was similar in the three culture media and two gas phases tested ranging from 44.8% (1:1 IMDM:NCTC + 5% CO2: 95% N2) to 56.7% (L-15 + 5% CO2: 95% air). Percent molting in O. volvulus ranged from 0% (F12(K) + 5% CO2: 95% N2) to 33.3% (L-15 + 5% CO2: 95% N2). All media were supplemented with either 20% FCS or 20% horse serum. Molting by O. lienalis occurred on days 2-5 in culture. Molting by O. volvulus was observed as early as day 5 and as late as day 10. Incomplete casting of the third-stage cuticle was frequently observed in O. volvulus. Larvae of both species entered a lethargus 24-48 hours prior to the onset of molting. Maximum survival in culture was 42 days for O. lienalis and 25 days for O. volvulus. Significant growth of larval O. lienalis was noted early in the culture period, but neither species continued development to the fifth stage.
在各种无细胞体外系统中，观察到旋盘尾丝虫和链尾丝虫的三期幼虫蜕变为四期幼虫。在所测试的三种培养基和两种气相条件下，链尾丝虫完成蜕皮的百分比相似，范围从44.8%（1:1 IMDM:NCTC + 5% CO₂: 95% N₂）到56.7%（L-15 + 5% CO₂: 95% 空气）。旋盘尾丝虫的蜕皮百分比范围从0%（F12(K) + 5% CO₂: 95% N₂）到33.3%（L-15 + 5% CO₂: 95% N₂）。所有培养基均添加了20% 的胎牛血清或20% 的马血清。链尾丝虫在培养的第2至5天发生蜕皮。旋盘尾丝虫最早在第5天观察到蜕皮，最晚在第10天。在旋盘尾丝虫中经常观察到三期表皮的不完全蜕落。两种丝虫的幼虫在蜕皮开始前24至48小时进入休眠期。链尾丝虫在培养中的最长存活时间为42天，旋盘尾丝虫为25天。在培养早期，链尾丝虫幼虫有显著生长，但两种丝虫均未继续发育到五期。
