Park M M
Pahlavi Med J. 1978 Jan;9(1):1-26.
The in vitro cytotoxic effect of anti Sarcoma I sera tumor cells in the presence of an exogenous source of complement was studied. The immune sera were obtained from xenogeneic and allogeneic animals, rabbits and mice, after injections with viable Sarcoma I tumor cells. Antibodies against mouse specific antigens were removed from the rabbit serum, by extensive absorption with mouse erythrocytes and lymphocytes. The absorbed serum was cytotoxic for Sarcoma I tumor cells and did not show any cytotoxicity for A/Jax lymphocytes. The antiserum was purified by an immunoabsorbent column using G-100 Sephadex conjugated with Sarcoma I tumor cell stroma. The eluted, purified antibodies were cytotoxic in vitro. Immunoelectrophoresis and immunodiffusion tests showed the presence of immunoglobulin in the purified antibody preparation. There was a hundred-fold increase of the specific activity of the purified antibodies as compared to the non-purified serum.
研究了在存在外源性补体的情况下抗肉瘤I血清对肿瘤细胞的体外细胞毒性作用。免疫血清取自异种和同种动物（兔子和小鼠），这些动物在注射活的肉瘤I肿瘤细胞后获得。通过用小鼠红细胞和淋巴细胞大量吸收，从兔血清中去除了针对小鼠特异性抗原的抗体。吸收后的血清对肉瘤I肿瘤细胞具有细胞毒性，而对A/Jax淋巴细胞没有任何细胞毒性。抗血清通过使用与肉瘤I肿瘤细胞基质偶联的G-100葡聚糖凝胶免疫吸附柱进行纯化。洗脱并纯化后的抗体在体外具有细胞毒性。免疫电泳和免疫扩散试验表明纯化的抗体制剂中存在免疫球蛋白。与未纯化的血清相比，纯化抗体的比活性提高了一百倍。
