Terman D S, Stewart I, Tavel A, Kirch D
Cancer Res. 1975 Jul;35(7):1761-6.
Studies of the mouse C-1300 neuroblastoma were undertaken in order to isolate tumor-specific antibodies and harness them for detection of tumors in vivo. Preliminary investigations demonstrated the strain-growth specificity of the neuroblastoma in A/Jax male mice and established the requirement for tumor viability for successful adoptive passage. Intradermally passaged tumor permitted extended survival of mice so that serum could be sampled at intervals for the presence of tumor-specific antibodies. By means of an indirect radioimmunoassay with glutaraldehyde-fixed identified in the serum of tumor-bearing hosts 6 days after inoculation, with a steady increase in antibody levels observed through Day 22. An eluate in which immunoglobulin G antibodies were identified by immunoelectrophoresis was obtained from purified tumor cells by acid buffer incubation. These antibodies were labeled with 125-I, absorbed with normal tissues, and injected into tumor-bearing mice. A selectively collimated single-probe isotope localization was positioned over the intradermal tumor, while the rest of the animal was shielded with lead. With this device, 125-neuroblastoma eluate was significantly taken up in the neuroblastoma but not in the mouse head or in a reticulum cell sarcoma control. Increasing uptake of MOPC 141 125-I-immunoglobulin G was not observed in either tumor. These studies suggest that the mouse neuroblastoma may provide a source of tumor-specific, antibodies and that, with sensitive monitoring devices, these antibodies may be utilized to localize occult neoplastic tissue in vivo.
为了分离肿瘤特异性抗体并利用它们在体内检测肿瘤，对小鼠C-1300神经母细胞瘤进行了研究。初步研究证明了神经母细胞瘤在A/Jax雄性小鼠中的品系生长特异性，并确定了肿瘤存活对于成功过继传代的必要性。皮内传代的肿瘤使小鼠存活期延长，从而可以定期采集血清以检测肿瘤特异性抗体的存在。通过间接放射免疫测定法，在接种后6天在荷瘤宿主的血清中检测到了用戊二醛固定的抗体，并且在第22天之前观察到抗体水平稳步上升。通过用酸性缓冲液孵育从纯化的肿瘤细胞中获得了一种洗脱液，通过免疫电泳鉴定其中含有免疫球蛋白G抗体。这些抗体用125I标记，用正常组织吸收，然后注射到荷瘤小鼠体内。一个选择性准直的单探针同位素定位仪放置在皮内肿瘤上方，而动物的其余部分用铅屏蔽。使用该装置，125I标记的神经母细胞瘤洗脱液在神经母细胞瘤中显著摄取，但在小鼠头部或网状细胞肉瘤对照中未摄取。在两种肿瘤中均未观察到MOPC 141 125I标记的免疫球蛋白G摄取增加。这些研究表明，小鼠神经母细胞瘤可能提供肿瘤特异性抗体的来源，并且通过灵敏的监测设备，这些抗体可用于在体内定位隐匿性肿瘤组织。
