Lee K S, Gartner L M, Vaisman S L
Pediatr Res. 1978 Apr;12(4 Pt 1):301-7. doi: 10.1203/00006450-197804000-00011.
Four methods for the measurement of bilirubin-albumin binding have been compared. Three of these, the fluorescent dye binding (Direct Yellow 7), Sephadex G-25 column chromatography, and the 2-(4-hydroxyazobenzene)benzoic acid (HBABA) dye binding methods demonstrate significant correlations of measured binding capacities for bilirubin over a range of bilirubin/albumin molar ratios. All three methods concurred in the demonstration that fresh adult human sera had a higher molar albumin binding capacity for bilirubin than the purified human serum albumin preparations. The fluorescent dye binding and Sephadex column methods agreed most closely in defining presumed deficiency in binding capacity. The HBABA dye binding method was less consistent and appeared to measure non-bilirubin binding sites on albumin in addition to bilirubin binding sites. The fourth method, the saturation index, yielded highly variable results as compared with the other methods because of an inherent excessive risk of laboratory error.
对四种测量胆红素与白蛋白结合的方法进行了比较。其中三种方法，即荧光染料结合法（直接黄7）、葡聚糖凝胶G - 25柱色谱法和2 -（4 - 羟基偶氮苯）苯甲酸（HBABA）染料结合法，在一系列胆红素/白蛋白摩尔比范围内，所测得的胆红素结合能力显示出显著相关性。所有这三种方法均证实，新鲜成人血清对胆红素的摩尔白蛋白结合能力高于纯化的人血清白蛋白制剂。在确定假定的结合能力缺陷方面，荧光染料结合法和葡聚糖凝胶柱法最为接近。HBABA染料结合法不太一致，除了胆红素结合位点外，似乎还测量了白蛋白上的非胆红素结合位点。与其他方法相比，第四种方法即饱和指数产生的结果高度可变，因为存在固有的实验室误差风险过高的问题。
