Chung Y H, Miyake H, Kamei C, Tasaka K
Agents Actions. 1984 Oct;15(3-4):137-42. doi: 10.1007/BF01972339.
Three methods were used to study the analgesic effect of intracerebral injection of histamine (Hi) on mice: the writhing test (acetic acid and phenylquinone), the electrical stimulation of the tail and the hot plate test. At doses higher than 2 micrograms, Hi inhibited the writhing syndrome significantly, and at doses of 10 micrograms or higher, Hi displayed a marked analgesic effect during both the electrical stimulation and hot plate methods. The saline injection produced only a negligible effect. Simultaneous application of Hi and 10 micrograms of diphenhydramine, pyrilamine or promethazine, apparently causing no analgesic effect from a single administration, caused a parallel shift of the dose-response curve of Hi to the right. ED50 of Hi was increased approximately 2, 2.8 and 3.8 times, respectively. However, cimetidine did not reveal any antagonistic effect on Hi-induced analgesia. Subcutaneously administered, 3 mg/kg of morphine augmented the analgesic effect of Hi. In accordance with this, pretreatment of naloxone (0.005 mg/kg) antagonized the analgesic action of Hi almost completely. When 5 mg/kg of leucine-enkephalin, less than the minimum effective dose, was given prior to Hi injection, the analgesic effect of Hi was enhanced. In addition, 10 and 20 micrograms of Hi increased the morphine analgesia markedly and parallel shifted the dose-response curve of morphine to the left.
采用三种方法研究脑室内注射组胺（Hi）对小鼠的镇痛作用：扭体试验（乙酸和苯醌）、电刺激尾部试验和热板试验。当剂量高于2微克时，Hi能显著抑制扭体反应；当剂量为10微克或更高时，Hi在电刺激和热板试验中均表现出明显的镇痛作用。注射生理盐水仅产生可忽略不计的效果。同时应用Hi和10微克苯海拉明、吡苄明或异丙嗪，单次给药时显然无镇痛作用，但导致Hi的剂量-反应曲线平行右移。Hi的半数有效量（ED50）分别增加约2倍、2.8倍和3.8倍。然而，西咪替丁对Hi诱导的镇痛作用未显示出任何拮抗作用。皮下注射3毫克/千克吗啡可增强Hi的镇痛作用。与此一致的是，纳洛酮（0.005毫克/千克）预处理几乎完全拮抗了Hi的镇痛作用。在注射Hi之前给予5毫克/千克亮氨酸脑啡肽（低于最小有效剂量），可增强Hi的镇痛作用。此外，10微克和20微克的Hi显著增强吗啡的镇痛作用，并使吗啡的剂量-反应曲线平行左移。
