Gresham C N, Confer A W, Bush L J, Rummage J A
Am J Vet Res. 1984 Nov;45(11):2227-30.
A survey of antibody to Pasteurella haemolytica and P multocida, using a fluorometric immunoassay, was conducted on sera collected from 264 dairy cattle from 3 herds. Serum antibody titers to P haemolytica were 0 to 270 with low titers (less than 25) seen in 48.1% of the cows and heifers. Serum antibody titers to P multocida were 0 to 380 and the frequency of distribution of these titers were more even than for P haemolytica. Mean serum antibody titers to P haemolytica were significantly (P less than 0.005) higher in cattle from an open dairy herd when compared with those from 2 closed herds. Antibody titers to these organisms was determined in 7 colostrum samples. Pasteurella haemolytica antibody titers varied, depending on the whey separation technique used. Passive transfer of colostrum-derived antibody in 5 neonatal calves resulted in a maximum mean serum antibody titer at 20 hours after birth for P haemolytica and at 8 hours after birth for P multocida. Serum titers were higher overall for P multocida than for P haemolytica. Serum titers for P haemolytica declined rapidly. A significant (P less than 0.05) increase in antibody to P multocida was observed at 5 days of age.
采用荧光免疫分析法，对来自3个牛群的264头奶牛采集的血清进行了溶血巴斯德菌和多杀性巴氏杆菌抗体检测。溶血巴斯德菌的血清抗体滴度为0至270，48.1%的母牛和小母牛抗体滴度较低（低于25）。多杀性巴氏杆菌的血清抗体滴度为0至380，这些滴度的分布频率比溶血巴斯德菌更均匀。与来自2个封闭牛群的牛相比，开放式奶牛群的牛对溶血巴斯德菌的平均血清抗体滴度显著更高（P<0.005）。对7份初乳样本检测了这些微生物的抗体滴度。溶血巴斯德菌的抗体滴度因所用的乳清分离技术而异。5头新生犊牛初乳来源抗体的被动转移导致溶血巴斯德菌在出生后20小时达到最高平均血清抗体滴度，多杀性巴氏杆菌在出生后8小时达到最高平均血清抗体滴度。多杀性巴氏杆菌的血清滴度总体上高于溶血巴斯德菌。溶血巴斯德菌的血清滴度迅速下降。在5日龄时观察到多杀性巴氏杆菌抗体显著增加（P<0.05）。
