Confer A W, Fox J C, Newman P R, Lawson G W, Corstvet R E
Can J Comp Med. 1983 Jan;47(1):37-42.
A rapid, simple fluorometric method is described for measuring antibody to Pasteurella haemolytica in sera of cattle. Various antigen preparations were compared for the test including live, formalin-killed and phenol-killed P. haemolytica. A preparation composed of formalin-killed organisms from a 22 hour culture gave consistent results and was used in the studies. The test was reproduciable with percent coefficients of variation for fluorescent signal unit values on ten or more replicate samples ranging from 5.7 to 28.0. Sera from calves vaccinated by aerosol exposure to live P. haemolytica had up to a five-fold increase in antibody titer as measured by the flurometric method test during a 21 day period. Fluorometric method titers were comparable to those obtained by the indirect bacterial agglutination test. There was no seroconversion to P. haemolytica in calves vaccinated by aerosol exposure of P. multocida. The major advantages of the fluorometric method test over conventional methods are that the assay does not require serial dilutions of serum samples and thus limits time and effort to determine antibody titers.
本文描述了一种快速、简便的荧光测定法，用于检测牛血清中溶血巴斯德氏菌抗体。对包括活的、经福尔马林灭活和经苯酚灭活的溶血巴斯德氏菌在内的各种抗原制剂进行了检测比较。一种由来自22小时培养物的福尔马林灭活菌体制成的制剂给出了一致的结果，并用于这些研究。该检测方法具有可重复性，对十个或更多重复样本的荧光信号单位值的变异系数百分比在5.7%至28.0%之间。通过气溶胶暴露于活的溶血巴斯德氏菌而接种疫苗的犊牛血清，在21天期间，通过荧光测定法检测，抗体滴度最高增加了五倍。荧光测定法的滴度与通过间接细菌凝集试验获得的滴度相当。通过气溶胶暴露于多杀巴斯德氏菌而接种疫苗的犊牛，未出现对溶血巴斯德氏菌的血清转化。荧光测定法相对于传统方法的主要优点在于，该检测不需要对血清样本进行系列稀释，因此限制了测定抗体滴度所需的时间和精力。
