Gershwin L J, Olsen C L
Am J Vet Res. 1984 Dec;45(12):2511-7.
The humoral response of cattle to ovalbumin (OA), a nonenvironmental well-defined antigen, was studied. During 9 weeks of aerosolization, weekly serum and nasal secretion concentrations of immunoglobulin (Ig)G1, IgG2, IgM, IgA, and IgE were determined by enzyme-linked immunosorbent assay (ELISA) for OA specific antibody. Data from 3 calves given aerosol OA were compared and contrasted with data from 3 calves given aerosol saline solution and 1 calf given parenteral OA. The presence of cytotropic (skin sensitizing) antibody was evaluated during weeks 6 and 9 by direct skin testing with OA. A humoral response was induced in all 3 calves given aerosol OA. Serum IgG1 and IgG2 titers reached a maximum of 64,000 and 2,000, respectively, in calves given aerosol OA compared with 521,000 and 16,000, respectively, in the calf given parenteral OA. The ELISA did not detect an OA-specific IgM response. In contrast, all 3 calves given aerosol OA had serum IgA concentrations that increased to a peak by week 9. The mean IgA absorbance value for the 3 calves given aerosol OA was slightly greater than 5 times that of the calf given parenteral OA. Similarly, nasal secretions from calves given aerosolized OA had absorbance values that were 15-fold greater than that from the calf given parenteral OA. Calves given aerosol OA had antigen-specific IgE responses during weeks 6 to 8. The ELISA results were compared with results of passive cutaneous anaphylaxis tests. The presence of skin-sensitizing antibody was indicated by positive skin tests in the calves given aerosol OA and the calf given parenteral OA by week 9.
研究了牛对卵清蛋白（OA）这种非环境性明确抗原的体液免疫反应。在9周的雾化过程中，每周通过酶联免疫吸附测定（ELISA）来测定血清和鼻分泌物中针对OA特异性抗体的免疫球蛋白（Ig）G1、IgG2、IgM、IgA和IgE的浓度。将3头接受雾化OA的小牛的数据与3头接受雾化盐溶液的小牛以及1头接受肠胃外OA的小牛的数据进行比较和对比。在第6周和第9周通过用OA进行直接皮肤试验来评估亲细胞性（皮肤致敏）抗体的存在情况。所有3头接受雾化OA的小牛都诱导出了体液免疫反应。接受雾化OA的小牛血清IgG1和IgG2滴度分别最高达到64,000和2,000，相比之下，接受肠胃外OA的小牛血清IgG1和IgG2滴度分别为521,000和16,000。ELISA未检测到OA特异性IgM反应。相比之下，所有接受雾化OA的3头小牛血清IgA浓度在第9周时均升高至峰值。接受雾化OA的3头小牛血清IgA吸光度平均值略大于接受肠胃外OA的小牛的5倍。同样，接受雾化OA的小牛鼻分泌物的吸光度值比接受肠胃外OA的小牛高15倍。接受雾化OA的小牛在第6至8周出现了抗原特异性IgE反应。将ELISA结果与被动皮肤过敏试验结果进行了比较。到第9周时，接受雾化OA的小牛和接受肠胃外OA的小牛皮肤试验呈阳性，表明存在皮肤致敏抗体。
