来自藤黄微球菌的参与自发DNA损伤切除修复初始步骤的酶：尿嘧啶-DNA-糖苷酶和脱嘌呤内切核酸酶。
Enzymes from Micrococcus luteus involved in the initial steps of excision repair of spontaneous DNA lesions: uracil-DNA-glycosidase and apurinic-endonucleases.
作者信息
Tomlin N V, Aprelikova O N, Barenfeld L S
出版信息
Nucleic Acids Res. 1978 Apr;5(4):1413-28. doi: 10.1093/nar/5.4.1413.
Abstract
Uracil-DNA-glycosidase that releases free uracil from single-stranded or double-stranded deaminated DNA and poly d(A-U) has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 16,000 and can be separated from uracil-endonuclease and endonucleases (AP-endonucleases) specific for apurinic and apyrimidinic sites. Uracil-DNA-glycosidase does not act on guanine residues opposite uracil in double-stranded DNA and on xanthine in deaminated DNA. The glycosidase generates apyrimidinic sites which can serve as substrate sites for different AP-endonucleases from M. luteus.
摘要
已从藤黄微球菌中部分纯化出尿嘧啶-DNA-糖苷酶，该酶可从单链或双链脱氨基DNA以及聚d(A-U)中释放游离尿嘧啶。该酶的分子量约为16,000，可与尿嘧啶内切核酸酶以及对无嘌呤和无嘧啶位点具有特异性的内切核酸酶（AP-内切核酸酶）分离。尿嘧啶-DNA-糖苷酶对双链DNA中与尿嘧啶相对的鸟嘌呤残基以及脱氨基DNA中的黄嘌呤不起作用。该糖苷酶产生无嘧啶位点，这些位点可作为藤黄微球菌不同AP-内切核酸酶的底物位点。
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