Aue W P, Roufosse A H, Glimcher M J, Griffin R G
Biochemistry. 1984 Dec 4;23(25):6110-4. doi: 10.1021/bi00320a032.
Phosphorus-31 NMR spectra have been obtained from a variety of synthetic, solid calcium phosphate mineral phases by magic angle sample spinning. The samples include crystalline hydroxyapatite, two type B carbonatoapatites containing 3.2 and 14.5% CO3(2-), respectively, a hydroxyapatite in which approximately 12% of the phosphate groups are present as HPO4(2-), an amorphous calcium phosphate, monetite, brushite, and octacalcium phosphate. Spectra were observed by the standard Bloch decay and cross-polarization techniques, as well as by a dipolar suppression sequence, in order to distinguish between protonated and unprotonated phosphate moieties. The spectra of the synthetic calcium phosphates provide basic information that is essential for interpreting similar spectra obtained from bone and other calcified tissues.
通过魔角样品旋转技术，已从多种合成的固体磷酸钙矿物相中获得了磷-31核磁共振谱。样品包括结晶羟基磷灰石、两种分别含有3.2%和14.5%碳酸根离子（CO3(2-)）的B型碳酸磷灰石、约12%的磷酸基团以磷酸氢根离子（HPO4(2-)）形式存在的羟基磷灰石、无定形磷酸钙、磷酸二氢钙、磷酸氢钙二水合物和磷酸八钙。通过标准的布洛赫衰减和交叉极化技术以及偶极抑制序列来观察谱图，以便区分质子化和未质子化的磷酸基团。合成磷酸钙的谱图提供了基本信息，这对于解释从骨骼和其他钙化组织获得的类似谱图至关重要。
