Ohno T, Yamada M
Biomed Pharmacother. 1984;38(7):337-43.
Clonal growth of the normal human lung fibroblasts, IMR-90, is stimulated efficiently by utilizing feeder fibroblasts. Under the sparse culture conditions, IMR-90 cells located close to the feeder cells formed colonies with a higher efficiency than those isolated from the feeders within the same dish. The IMR-90 cells in direct contact with the 3T3 cell feeders, as monitored by metabolic cooperation, showed a faster entrance into S phase than did the IMR-90 cells isolated from the feeders. The isolated cells, even when they remained closer to the feeder cells, entered S phase later than those of the IMR-90 cells making direct contact with the feeders with long pseudopods. Medium- and dish-surface-conditioning with the feeders resulted in marginal improvement of the clonal growth. These observations suggest that contact stimulation of growth occurs in sparse cultures of normal human lung fibroblasts.
通过利用饲养层成纤维细胞，可有效刺激正常人肺成纤维细胞IMR - 90的克隆生长。在稀疏培养条件下，靠近饲养层细胞的IMR - 90细胞形成集落的效率高于同一培养皿中与饲养层细胞分离的细胞。通过代谢合作监测发现，与3T3细胞饲养层直接接触的IMR - 90细胞进入S期的速度比从饲养层分离的IMR - 90细胞更快。即使分离的细胞与饲养层细胞保持较近的距离，它们进入S期的时间也比通过长伪足与饲养层直接接触的IMR - 90细胞晚。饲养层对培养基和培养皿表面的预处理使克隆生长略有改善。这些观察结果表明，正常人肺成纤维细胞的稀疏培养中存在生长的接触刺激。
