Stathakos D, Psarras S, Kletsas D
Laboratory for Enzyme Research, National Center for Scientific Research Demokritos, Athens, Greece.
Cell Biol Int. 1993 Jan;17(1):55-64. doi: 10.1006/cbir.1993.1005.
The concerted action of TGF-beta and PDGF on a diploid human embryonic lung fibroblast cell strain (Flow 2002) grown in an homologous environment is investigated here. In sparse cultures, TGF-beta stimulates DNA synthesis over a broad concentration range (0.1-10 ng/ml). Furthermore, it acts in synergism with PDGF, a phenomenon which persists also during in vitro aging of the cells. Preincubation of TGF-beta with the fibroblasts up to 12 hours reduces the subsequent PDGF binding to the cells, while prolonged preincubation restores PDGF binding to control levels. Finally, in cultures of higher cell densities, TGF-beta ceases to stimulate DNA synthesis, whereas PDGF continues even at cell confluency, retains its stimulatory activity suggesting different roles for the two growth factors during the wound healing process.
本文研究了转化生长因子-β（TGF-β）和血小板衍生生长因子（PDGF）在同源环境中培养的二倍体人胚肺成纤维细胞系（Flow 2002）上的协同作用。在稀疏培养中，TGF-β在较宽的浓度范围（0.1 - 10 ng/ml）内刺激DNA合成。此外，它与PDGF协同作用，这种现象在细胞的体外老化过程中也持续存在。将TGF-β与成纤维细胞预孵育长达12小时会降低随后PDGF与细胞的结合，而长时间预孵育可使PDGF结合恢复到对照水平。最后，在细胞密度较高的培养物中，TGF-β停止刺激DNA合成，而PDGF即使在细胞汇合时仍继续起作用，保留其刺激活性，这表明这两种生长因子在伤口愈合过程中具有不同的作用。
