Bernier J, Reuter A, Vrindts-Gevaert Y, Franchimont P, Pouillart P, Bretaudeau B, Falcoff E, Magdelenat H
Bull Cancer. 1984;71(5):405-11.
Using a radioimmunoassay for human leukocyte interferon (IFN alpha), pharmacokinetic studies were carried out in twelve cancer patients given sequential intramuscular injections of Hu IFN alpha 2. Even though individual monitoring of serum IFN titers emphasized, for a given dose, marked quantitative variations of the observed maximum concentrations, their mean values were found to be dose-dependent (358 +/- 167 U/ml at 30.10(6) U and 1044 +/- 599 U/ml at 100.10(6) U doses). Comparison with bioassay results showed that IFN activities measured in sera were of the same order of magnitude as those calculated from radioimmunoassay standard curves. Data obtained from this series on observed peak time, half-life value and serum concentrations were consistent with those reported by the other groups using recombinant leukocyte interferon in clinical trial. Therefore, radioimmunoassay is an useful method for routinely assaying IFN alpha used either as antitumour or antivirus agent because of its high sensitivity (4 U/ml) and its simplicity.
采用人白细胞干扰素（IFNα）放射免疫分析法，对12例接受Hu IFNα 2序贯肌肉注射的癌症患者进行了药代动力学研究。尽管针对给定剂量，血清IFN滴度的个体监测强调观察到的最大浓度存在明显的定量差异，但发现其平均值呈剂量依赖性（30×10⁶ U时为358±167 U/ml，100×10⁶ U剂量时为1044±599 U/ml）。与生物测定结果比较表明，血清中测得的IFN活性与根据放射免疫分析标准曲线计算的值处于同一数量级。从该系列获得的关于观察到的峰值时间、半衰期值和血清浓度的数据与其他组在临床试验中使用重组白细胞干扰素报告的数据一致。因此，放射免疫分析因其高灵敏度（4 U/ml）和简便性，是常规检测用作抗肿瘤或抗病毒药物的IFNα的有用方法。
