Bernier J, Reuter A, Vrindts-Gevaert Y, Franchimont P
J Nucl Med. 1984 Jul;25(7):765-72.
A radioimmunoassay for human leukocyte interferon alpha (alpha IFN) has been developed, using tracer produced by recombinant DNA (IFLrA) and polyclonal rabbit antiserum against partially purified leukocyte IFN (PP alpha IFN). Sensitivity was 4 units/ml using sequential saturation. Only lymphoblastoid IFN showed complete cross reactivity. Serum alpha IFN concentrations were measured in normal subjects and in patients with acute viral infections, bone and joint diseases, and malignancies. Some cases in the first two groups of patients had significantly elevated serum levels compared with controls. The pharmacokinetics of alpha IFN were studied in treated cancerous patients. Radioimmunoassay and biological assay gave similar and closely correlated results. Radioimmunoassay is thus a useful method for the routine assay of alpha IFN, especially in biological fluids containing low concentrations.
已开发出一种用于检测人白细胞干扰素α（α干扰素）的放射免疫测定法，该方法使用重组DNA产生的示踪剂（IFLrA）和针对部分纯化的白细胞干扰素（PPα干扰素）的兔多克隆抗血清。采用连续饱和法时，灵敏度为4单位/毫升。只有淋巴母细胞样干扰素显示出完全交叉反应性。对正常受试者以及患有急性病毒感染、骨和关节疾病及恶性肿瘤的患者测定了血清α干扰素浓度。与对照组相比，前两组患者中的一些病例血清水平显著升高。在接受治疗的癌症患者中研究了α干扰素的药代动力学。放射免疫测定法和生物测定法给出了相似且密切相关的结果。因此，放射免疫测定法是常规检测α干扰素的一种有用方法，尤其是在低浓度的生物体液中。
