一种针对血管紧张素II羧基末端的单克隆抗体。

A monoclonal antibody specific for the carboxy-terminus of angiotensin II.

作者信息

Nussberger J, Mudgett-Hunter M, Matsueda G, Haber E

出版信息

Hybridoma. 1984 Winter;3(4):373-6. doi: 10.1089/hyb.1984.3.373.

DOI:10.1089/hyb.1984.3.373

Abstract

A monoclonal antibody specific for the carboxy-terminus of angiotensin II was produced from the somatic cell fusion between SP2/0 myeloma cells and spleen cells from CAF/J mice immunized with angiotensin II selectively coupled to thyroglobulin. The subcloned line D5-7R2 was augmented in the ascites form and shown to be specific for angiotensin II (octapeptide) and shorter angiotensin peptides. This antibody, however, did not cross-react with angiotensin I (decapeptide). This selectivity will be important in clinical assays that measure angiotensin II in the presence of angiotensin I.

摘要

一种针对血管紧张素II羧基末端的单克隆抗体，是通过将SP2/0骨髓瘤细胞与用选择性偶联甲状腺球蛋白的血管紧张素II免疫的CAF/J小鼠的脾细胞进行体细胞融合而产生的。亚克隆系D5-7R2以腹水形式扩增，并显示对血管紧张素II（八肽）和较短的血管紧张素肽具有特异性。然而，这种抗体与血管紧张素I（十肽）不发生交叉反应。这种选择性在存在血管紧张素I的情况下测量血管紧张素II的临床检测中具有重要意义。