Steiner M, Frick J, Rovan E
Int J Androl. 1984 Dec;7(6):521-8. doi: 10.1111/j.1365-2605.1984.tb00809.x.
LDH isoenzyme activities of heart, liver and testis homogenates were measured in rats treated for 15 or 30 days with 30 mg gossypol acetic acid/kg body weight daily, a dose which severely suppressed sperm motility. No differences in LDH activities of the examined organs were found in comparison with control animals. LDH-X, the main LDH isoenzyme in spermatozoa and testicular germ cells from primary spermatocytes onwards was also unaffected. These results contrast with in vitro experiments on human, rabbit, and boar spermatozoa, purified bovine LDH-X and rat testis homogenate which show dose-dependent inhibition of LDH-X after incubation with gossypol. Our results therefore suggest that inhibition of LDH by gossypol is not the primary cause of sperm immotility.
对每日按30毫克棉酚乙酸/千克体重的剂量处理15天或30天的大鼠，测定其心脏、肝脏和睾丸匀浆的乳酸脱氢酶（LDH）同工酶活性，该剂量会严重抑制精子活力。与对照动物相比，在所检查器官的LDH活性方面未发现差异。从初级精母细胞开始，精子和睾丸生殖细胞中的主要LDH同工酶LDH-X也未受影响。这些结果与对人、兔和公猪精子、纯化的牛LDH-X和大鼠睾丸匀浆进行的体外实验形成对比，后者显示与棉酚孵育后LDH-X受到剂量依赖性抑制。因此，我们的结果表明棉酚对LDH的抑制不是精子运动能力丧失的主要原因。
