一些突触体肽和合成肽的质谱研究。

Mass spectrometric studies of some synaptosomal and synthetic peptides.

作者信息

Marnela K M, Hagen E A, Aasen A J, Loennechen T, Sydnes L K, Timonen M, Lähdesmäki P

出版信息

Int J Neurosci. 1984 Dec;25(1-2):123-30. doi: 10.3109/00207458408985594.

DOI:10.3109/00207458408985594

Abstract

Mass spectra were recorded using direct inlet and electron impact ionization from two synthetic peptides, N-acetyl-aspartyl-4-aminobutyric acid and gamma-glutamyl-taurine and a natural peptide, N-acetyl-aspartyl-glycyl-alanyl-aspartyl-serine, isolated from calf brain synaptosomes. The peptides were studied either underivatized or first acetylated with acetic acid anhydride and then permethylated with methyl iodide. The synthetic peptides were used to check the completeness of the derivatization reactions.

摘要

使用直接进样和电子轰击电离，记录了两种合成肽（N-乙酰天冬氨酰-4-氨基丁酸和γ-谷氨酰牛磺酸）以及从小牛脑突触体中分离出的天然肽（N-乙酰天冬氨酰-甘氨酰-丙氨酰-天冬氨酰-丝氨酸）的质谱。这些肽要么未衍生化，要么先用乙酸酐乙酰化，然后用碘甲烷进行全甲基化。合成肽用于检查衍生化反应的完整性。