Nazareth A, Joppich M, Abdel-Baky S, O'Connell K, Sentissi A, Giese R W
J Chromatogr. 1984 Nov 30;314:201-10. doi: 10.1016/s0021-9673(01)97734-9.
The pyrimidine bases cytosine, uracil and thymine, along with some analogues, are electrophore-labeled either with pentafluorobenzoyl chloride (PFBC), pentafluorophenylsulfonyl chloride (PPSC), or heptafluorobutyric anhydride. Subsequent alkylation is most successful for PFB-cytosine, PPS-uracil, and PPS-thymine. These same alkylated compounds also have the highest aqueous stability and respond most strongly by gas chromatography-electron-capture detection. One of these derivatives, determined to be N4-PFB-1,3-dimethylcytosine by authentic synthesis, and its 5-methyl analogue, can be detected with good precision down to the 100-fg level. Poor reproducibility is encountered at the 10-fg level.
嘧啶碱胞嘧啶、尿嘧啶和胸腺嘧啶，以及一些类似物，用五氟苯甲酰氯（PFBC）、五氟苯磺酰氯（PPSC）或七氟丁酸酐进行电泳标记。随后的烷基化对PFB - 胞嘧啶、PPS - 尿嘧啶和PPS - 胸腺嘧啶最为成功。这些相同的烷基化化合物在水中也具有最高的稳定性，并且在气相色谱 - 电子捕获检测中响应最强。通过纯合成确定其中一种衍生物为N4 - PFB - 1,3 - 二甲基胞嘧啶，其5 - 甲基类似物在低至100 fg水平时能够以良好的精密度检测到。在10 fg水平时重现性较差。
