用五氟苯甲酰氯和硫酸二甲酯对胞嘧啶进行痕量衍生化。
Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
作者信息
Fisher D H, Adams J, Giese R W
出版信息
Environ Health Perspect. 1985 Oct;62:67-71. doi: 10.1289/ehp.856267.
Abstract
Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%.
摘要
目前正在开发一些方法，用于衍生化痕量的DNA加合物，以便最终通过气相色谱技术进行测定。高压液相色谱法用于优化测定胞嘧啶的合适衍生化反应。单瓶反应方案包括用亲电的五氟苯甲酰氯进行酰化，然后用硫酸二甲酯进行烷基化。目前，以50纳摩尔胞嘧啶开始的该反应的总产率为59±4.6%。
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本文引用的文献
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Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.胞嘧啶和5-甲基胞嘧啶的甲基化全氟酰基衍生物的气相色谱-质谱特征
J Chromatogr. 1984 Nov 30;314:211-7. doi: 10.1016/s0021-9673(01)97735-0.
2
Electrophore-labeling and alkylation of standards of nucleic acid pyrimidine bases for analysis by gas chromatography with electron-capture detection.用于通过电子捕获检测气相色谱法分析的核酸嘧啶碱基标准品的电泳标记和烷基化。
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