Knoll E, Wisser H
J Clin Chem Clin Biochem. 1984 Nov;22(11):741-9.
This review describes experiments to produce antibodies towards the catecholamines and some catecholamine metabolites with the intention of developing radioimmunological methods. First, attempts to produce antibodies to the catecholamines themselves are described. In the course of synthesis of immunogens of catecholamines, it was necessary to exercise special care - e.g. the introduction of protecting groups - owing to the great susceptibility of the catechol structure to oxidation. Despite many efforts, only one working group (Miwa et al.) has reported in a series of papers the successful production of antibodies to catecholamines, but they did not develop a radioimmunoassay. In contrast, antibodies to some metabolites of the catecholamines - such as 3,4-dimethoxyphenylethylamine, the 3-O-methylated catecholamines (normetanephrine, metaneprine, and 3-methoxytyramine) as well as 3-methoxy-4-hydroxyphenylethyleneglycol - have been produced, whose avidity and specificity were high enough to permit the development of sensitive radioimmunoassays.
本综述描述了为开发放射免疫分析方法而制备针对儿茶酚胺及其某些代谢产物的抗体的实验。首先，介绍了制备针对儿茶酚胺本身的抗体的尝试。在儿茶酚胺免疫原的合成过程中，由于儿茶酚结构极易氧化，因此有必要格外小心，例如引入保护基团。尽管付出了诸多努力，但只有一个研究小组（Miwa等人）在一系列论文中报道成功制备了针对儿茶酚胺的抗体，但他们并未开发出放射免疫分析方法。相比之下，已经制备出了针对儿茶酚胺某些代谢产物的抗体，如3,4 - 二甲氧基苯乙胺、3 - O - 甲基化儿茶酚胺（去甲变肾上腺素、变肾上腺素和3 - 甲氧基酪胺）以及3 - 甲氧基 - 4 - 羟基苯乙二醇，其亲和力和特异性足以支持开发灵敏的放射免疫分析方法。
