Keeton T K, Krutzsch H, Lovenberg W
Science. 1981 Feb 6;211(4482):586-8. doi: 10.1126/science.7455697.
Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites including MOPEG-sulfate (333 nanograms each).
在给兔子注射与牛甲状腺球蛋白偶联的去甲肾上腺素代谢物3-甲氧基-4-羟基苯乙二醇（MOPEG）衍生物后，产生了特异性结合MOPEG的抗体。用这种抗血清设计了一种灵敏的放射免疫测定法，在最终抗体稀释度为1:180时，低至0.5纳克的MOPEG都能被准确测量。该抗体似乎对MOPEG具有特异性，因为去甲肾上腺素、肾上腺素、多巴胺、5-羟色胺或它们的主要代谢物（包括硫酸MOPEG，每种333纳克）都不能将氚标记的MOPEG从抗体上置换下来。
