Garth H, Zoch E
J Clin Chem Clin Biochem. 1984 Nov;22(11):769-72.
The enzymatic deamination of adenosine to inosine produces ammonia, which causes a pH-increase of the reaction mixture. The pH-stat method is based on the continuous addition of protons to keep the pH at a constant value. The theoretical principles are discussed. The quantitative limits of the assay and the effect of changing the pH were investigated. A correction factor was derived and calculated for the pH range 6.4 to 8.5. This sensitive method allows the continuous recording of the adenosine deaminase activity in strongly coloured or turbid biological samples.
腺苷酶促脱氨生成肌苷会产生氨，这会导致反应混合物的pH值升高。pH稳态法基于持续添加质子以保持pH值恒定。文中讨论了其理论原理。研究了该测定方法的定量限度以及pH值变化的影响。推导并计算了6.4至8.5 pH范围内的校正因子。这种灵敏的方法能够连续记录深色或浑浊生物样品中的腺苷脱氨酶活性。
