Carson D A, Goldblum R, Seegmiller J E
J Immunol. 1977 Jan;118(1):270-3.
We have developed a quantitative immunoassay for the measurement of human adenosine deaminase that requires neither purified enzyme nor monospecific antibody. With this technique, cultured fibroblasts from three immunodeficient patients were shown to contain adenosine deaminase as detected both catalytically and immunochemically. In one patient the level of immunoreactive enzyme exceeded the amount of catalytically active adenosine deaminase, suggesting a structural alteration in the enzyme molecule.
我们已经开发出一种用于测量人腺苷脱氨酶的定量免疫测定法，该方法既不需要纯化的酶，也不需要单特异性抗体。通过这项技术，对三名免疫缺陷患者的培养成纤维细胞进行检测，结果显示，无论是通过催化检测还是免疫化学检测，这些细胞都含有腺苷脱氨酶。在一名患者中，免疫反应性酶的水平超过了催化活性腺苷脱氨酶的量，这表明酶分子发生了结构改变。
