Jan M, Picaper G
Neurochirurgie. 1984;30(6):373-7.
In the first part: the charcoal-assay method was briefly described (sensitivity: PR = 10 fmol/mg protein, ER = 5 fmol/mg protein), and criteria for the validity of this method were analysed: sufficient amount of tumor (150-200 mg) minimum protein concentration should be 2 mg/ml choice of a suitable labeled hormone plotting of Scatchard curve with at least 4 aligned points and systematic control of the dissociation constant (Kd) systematic assay of a reference cytosol. In the second part: the results were analysed and compared with those of various authors: PR were positive in 13 out of 23 determinations (56,5%), 12-230 fmol/mg protein (mean: 93 fmol/mg protein) ER were positive in 2 out of 25 determinations (8%), 5 and 16 fmol/mg protein. Only one tumor showed positive ER and PR and no significant difference was shown between ER and PR results on male and female patients. In conclusion, the physiopathological significance of ER and PR is discussed and a new approach to treatment of some meningiomas is mentioned.
简要描述了活性炭测定法（灵敏度：孕激素受体（PR）=10飞摩尔/毫克蛋白，雌激素受体（ER）=5飞摩尔/毫克蛋白），并分析了该方法有效性的标准：足够量的肿瘤组织（150 - 200毫克），最低蛋白浓度应为2毫克/毫升，选择合适的标记激素，绘制至少有4个对齐点的Scatchard曲线，系统控制解离常数（Kd），对参考细胞质进行系统测定。在第二部分：分析结果并与其他作者的结果进行比较：在23次测定中有13次PR呈阳性（56.5%），12 - 230飞摩尔/毫克蛋白（平均：93飞摩尔/毫克蛋白）；在25次测定中有2次ER呈阳性（8%），5和16飞摩尔/毫克蛋白。只有一个肿瘤显示ER和PR均为阳性，且男性和女性患者的ER和PR结果之间无显著差异。总之，讨论了ER和PR的生理病理学意义，并提及了一种治疗某些脑膜瘤的新方法。
