Mertsalova L V, Luk'ianova L D
Tsitologiia. 1984 Jun;26(6):713-8.
A method of obtaining a peritoneal macrophage enriched population on a slide in a special chamber has been worked out and tested. It has been shown that such cells keep specific morphological and functional peculiarities, including the ability to phagocytosis. They are in active state and can be utilized for studying the biological oxidation processes. A relative activity of oxidative metabolism of key enzymes has been evaluated. The possibility of studying the respiratory chain activity in these cells was shown by polarographic and fluorescent methods. Alternative oxygen-dependent systems were investigated. It was determined that NADPH-oxidase of peritoneal macrophages attached to a slide, responsible for "the respiratory burst" formation and defining their bactericidal properties and peroxide generating ability, is in the active state.
一种在特殊腔室的载玻片上获得富集腹膜巨噬细胞群体的方法已经研发并经过测试。结果表明，此类细胞保持着特定的形态和功能特性，包括吞噬能力。它们处于活跃状态，可用于研究生物氧化过程。已评估关键酶氧化代谢的相对活性。通过极谱法和荧光法显示了研究这些细胞中呼吸链活性的可能性。对替代的氧依赖性系统进行了研究。已确定附着在载玻片上的腹膜巨噬细胞的NADPH氧化酶处于活跃状态，该酶负责“呼吸爆发”的形成，并决定其杀菌特性和过氧化物生成能力。
